Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Lipid topology and electrostatic interactions underpin lytic activity of linear cationic antimicrobial peptides in membranes

doi: 10.1073/pnas.1704489114

Figure Lengend Snippet: (A) Plan view of the device with the following parts labeled. (A1) Flow channel. (A2) Peptide channel. (A3) Wash channel. (A4) Electroformation chamber. (A5) Microtrap array chamber. (B) A mammalian biomimetic GUV (system A) with the liquid-disordered DOPC domain visualized with 0.1 mol% 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE)-rhodamine (red) and the liquid-ordered domain visualized using 0.5 mol% cholesterol-TopFluor (green). (C) SEM image of the microarray traps. (D) Vesicles trapped within the microarray, encapsulating 3 kDa dextran-AlexaFluor488. (Scale bar: 100 µm.)

Article Snippet: An advantage of this approach is the greatly increased experimental throughput over conventional techniques, which typically study only one GUV at a time. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. S1. caption a7 ( A ) Plan view of the device with the following parts labeled. ( A1 ) Flow channel. ( A2 ) Peptide channel. ( A3 ) Wash channel. ( A4 ) Electroformation chamber. ( A5 ) Microtrap array chamber. ( B ) A mammalian biomimetic GUV (system A) with the liquid-disordered DOPC domain visualized with 0.1 mol% 1,2-dipalmitoyl- sn -glycero-3-phosphoethanolamine (DPPE)-rhodamine (red) and the liquid-ordered domain visualized using 0.5 mol% cholesterol-TopFluor (green). ( C ) SEM image of the microarray traps. ( D ) Vesicles trapped within the microarray, encapsulating 3 kDa dextran-AlexaFluor488. (Scale bar: 100 μm.)

Techniques: Labeling, Microarray

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Lipid topology and electrostatic interactions underpin lytic activity of linear cationic antimicrobial peptides in membranes

doi: 10.1073/pnas.1704489114

Figure Lengend Snippet: (A) The normalized fluorescence intensity (NFI) function as a measure of the dye efflux of a system C GUV exposed to 1 µM LCAMP m2a. The trace displays multiple changes in leakage dynamics. (B) The −Ln(NFI) plot of the same trace shown in A. (C) This plot shows an example of the continuous change in membrane apparent permeability over the course of the entire leakage process from the data presented in A (as described in the SI Materials and Methods). D–G are the apparent membrane permeability histograms of (D) system A (mammalian biomimetic membrane) exposed to 1 µM m2a, which show the presence of distinct characteristic membrane flux rates or equivalently leakage characteristic times (τ; top axis). (E) System C (bacterial biomimetic) vesicles exposed to 1 µM m2a. (F) System A exposed to 1 µM melittin featuring the presence of flux groupings, although less evident than the case with m2a. (G) System C GUVs exposed to 1 µM melittin.

Article Snippet: An advantage of this approach is the greatly increased experimental throughput over conventional techniques, which typically study only one GUV at a time. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. S1. caption a7 ( A ) Plan view of the device with the following parts labeled. ( A1 ) Flow channel. ( A2 ) Peptide channel. ( A3 ) Wash channel. ( A4 ) Electroformation chamber. ( A5 ) Microtrap array chamber. ( B ) A mammalian biomimetic GUV (system A) with the liquid-disordered DOPC domain visualized with 0.1 mol% 1,2-dipalmitoyl- sn -glycero-3-phosphoethanolamine (DPPE)-rhodamine (red) and the liquid-ordered domain visualized using 0.5 mol% cholesterol-TopFluor (green). ( C ) SEM image of the microarray traps. ( D ) Vesicles trapped within the microarray, encapsulating 3 kDa dextran-AlexaFluor488. (Scale bar: 100 μm.)

Techniques: Fluorescence, Membrane, Permeability